Einstein International Journal Organization(EIJO)

Abstract

Background: Pseudomonas aeruginosa is a major opportunistic pathogen in cystic fibrosis, wound, and nosocomial infections, posing a serious burden to public health, due to its antibiotic resistance.  Antibiofilm agents serve as an essential tool in the fight against antibiotic resistance.

Objective: This study investigates the in vitro and in Silico Guided Identification of Anti-Biofilm Agents from Syzygium palghatense Against Pseudomonas aeruginosa

Methods: Leaves and bark of Syzygium palghatense were collected. Reference strains which were used to evaluate the antimicrobial activity of the methanol extract of leaves and bark of Syzygium palghatense were Aspergillus niger (ATCC 16404) and Candida albicans (ATCC 10231) for fungi and Staphylococcus aureus (ATCC 25923), Streptococcus mutans (MTCC 890) and Enterococcus fecalis (ATCC 2912) for Gram Gram-positive bacteria and E. coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853) for Gram Negative Bacteria. MIC values for filamentous fungi were determined using broth microdilution with methanol leaf and bark extracts serially diluted in DMSO (10–300 µg/ml). Antibiofilm activity against Pseudomonas aeruginosa was assessed, and molecular docking of bioactive compounds was performed against the target proteins of P. aeruginosa.

Results: The antimicrobial activity of methanol extracts from the leaves and bark of Syzygium palghatense varied against different pathogenic microorganisms. Pseudomonas aeruginosa shows higher activity. Syzygium palghatense extract has the ability to suppress the biofilm formation by Pseudomonas aeruginosa. Molecular docking shows a good docking score.

Conclusion: Methanol extracts of Syzygium palghatense demonstrated significant antimicrobial and antibiofilm activity against Pseudomonas aeruginosa, supported by favourable molecular docking results. These findings suggest that S. palghatense could serve as a potential natural source of anti-biofilm agents for managing P. aeruginosa-associated infections.

Keywords: Syzygium Palghatense, Pseudomonas Aeruginosa, Antimicrobial, Antibiofilm, Molecular Docking

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