A PHP Error was encountered

Severity: Notice

Message: Undefined offset: 0

Filename: models/Journals_model.php

Line Number: 97


File: /home/eijo/public_html/application/models/Journals_model.php
Line: 97
Function: _error_handler

File: /home/eijo/public_html/application/controllers/Journals.php
Line: 225
Function: get_journals

File: /home/eijo/public_html/index.php
Line: 292
Function: require_once

Einstein International Journal Organization(EIJO)

An International Journal House

Einstein International Journal Organization(EIJO)

Connecting People With Genius Thought

Einstein International Journal Organization(EIJO) is an international Genius Thought journals platform .
Seroprevalence and Associated Risk Factors for Seropositivity of Bovine Brucellosis at Wollega University Horro-Guduru Animal Breeding and Research Center

Author Names : Jirata Shiferaw1*, Alemayehu Fikadu2, Hika Waktole3  volume 1 issue 1
Article Overview


A cross-sectional study was carried out from November 2011 to March 2012 to determine the seroprevalence and associated risk factors for seropositivity of bovine brucellosis at Wollega University Horro-Guduru Animal Breeding and Research Center (WUHGABRC) in Horro-GuduruWollega Oromia Regional State. The study population comprised local (Horro) and cross (Horro-Jersy) breed. A total of 415 blood sample were collected from cattle above 1 year of age and were screened for brucella antibodies by Rose Bengal Plate Test (RBPT) and positive reactors were further tested by Complement Fixation Test (CFT). Out of 415 sera 4 (1%) were positive using RBPT. The positive sera were further confirmed using CFT in which 2 (0.5%) were positive. All positive sera were from female cattle. The results of current study revealed that seropositivity to bovine brucellosis was significantly higher in pregnant animals than non- pregnant (p<0.05). In contrast there was no statistically significant difference (p>0.05) in seroprevalence of bovine brucellosis between breeds, among different age groups and between sexes of study animals. Even though the prevalence of bovine brucellosis in the current study is low, it shouldn’t be ignored as nonexistent disease in the area where it may continue to become hazard to livestock industry and public consuming raw products of animal origin. Thus all peoples in general and high risk groups in particular need to be aware of zoonotic as well as economic importance of the disease, so as to adopt strategic control measures in order to reduce associated reproductive wastage and public health risk.

Keywords: Bovine Brucellosis, CFT, RBPT, WUHGABRC, Seroprevalence.


[1]. Anonymous, B. (2006): Brucellosis back ground prepared by Amer Vet Assoc, 44: 216-224.

[2]. Arimi,S. and Mangen,M. (2002): Brucellosis in Sub-Saharan Africa: Epidemiology, control and             impact. Veterinary Microbiology, 90:111-132.

[3]. Asfaw, Y., Molla., B., Zessin ,K. and Tegegn, A. (1998): Across sectional study on bovine        brucellosis and test performance in intra and peri-urban dairy production system in and around Addis Abeba. Bull Animhlth and prod Africa, 46: 217-224.  

[4]. Bekele, A., Molaa, B., Asfaw, Y. and Yigezu, T. (2000): Bovine brucellosis in ranches and farms          in south eastern Ethiopia. Bull.Anim. Hlth. Prodn. Afr.48: 13-17.

[5]. Berchovich, Z. (1998): The use of tests for diagnosis of brucellosis in cattle, 8th ed. Mosby,       Elsevier, Pp 286-289.

[6]. Birhanu, A. (2002): Animal health and poverty Reduction Strategies in proceeding of the 16th    Annual Conference of the Ethiopian veterinary association (EVA) held at Ghion Hotel     Addis Abeba, Ethiopia, The president of Ethiopian Veterinary Assciation.

[7]. Brew, S.D., Perret, L.L., Stack, J.J., Macmillan, A. P. and Staunton, N.J. (1999): Human             exposure to brucella recovered from a sea mammal, vet rec. 24: 483-490.

[8]. Crowford, R. p., Adams, L.G. and Williams, I.D. (1990): Relationship of fetal age at exposure of          pregnant heifers and Brucellaabourtus, Am. J. Vet.Res. 48: 755-782.

[9]. CSA. (2011): The Federal Democratic Republic of Ethiopia. Central Statistics Agency, Ethiopia.

[10]. Desalegn, F.(2008): Seroprevalence study of bovine brucellosis in Asella Gevermental dairy    farm,    Asella, Ethiopia, DVM thesis, Jimma university college of agriculture and veterinary  medicine Jimma Ethiopia.

[11]. Dohoo, I.R., Wright, P.F., Rucker, G.M., Semegh, B.S., Robertson, F.J. and Forbes, L.B. (1996):          A comparison of five serological tests for bovine brucelosisis .canada.J.Vet.Res.50:485-          493.

[12]. EMA, (1970): Ethiopian ministry of Agriculture. A review on animal health and production     factors. Sited from Dinka (995), Addis Abeba University, Faculity of Veterinary Medicine; Debrezeit, Ethiopia.

[13]. Endrias, Z. (1996): Seroprevalence study of bovine brucellosis in selected sites of sidama region,        DVM thesis, FVM, Addis Abeba University Debrezeit, Ethiopia.

[14]. FAO. (2003): Guidelines for coordinated human and animal brucellosis surveillance, FAO       animal production and health, Pp 156.

[15]. Fikadu, K. (1999): An epidemiological survey of bovine brucellosis in Amhara National          regional state.DVM Thesis, FVM, AAU, Debre Zeit, Ethiopia.

[16]. Faye, B., Castel, B., Lesnoff, M., Rutabinda, D. and Dhalwa, S. (2005): Tuberculosis and        brucellosis prevalence survey on dairy cattle in Mbarara milk basin (Uganda)      .prev.vet.Med. 64(4): 267-281.

[17]. Gebremariam, K. (1985): The Prevalence of bovine brucellosis in four different farms around             Addis Abeba, DVM thesis, FVM, AAU, DebreZeit, Ethiopai.

[18]. Hellman, E., Stack, C. and Baumann, M. (1984): Bovine brucellosis among two different         populations in Bahr el Ghazal Province of Southern Sudan. Trop.med.Parasitol. 36(2): 123 -126.

[19]. Hunduma, D. and Regasa, C. (2009): Seroprevalence study of bovine brucellosis in pastoral and         agro pastoral areas of east shoa zone, Oromia Rigional State Ethiopia.

[20]. Kagumba, M. and Nandokha, E. (1978): A survey of prevalence of bovine brucellosisin East Africa. Bull.anim.hlth.prod.26(3): 224-229.

[21]. Kebede, T., Ejeta, G. and Ameni, G. (2008): Seroprevalence of bovine brucellosis in smallholder farms in central Ethiopia (Wuchale- Jida district) Revue Vet. Med. 159(1):  3-9.

[22]. Lapraik, G. (1982): Latent bovine brucellosis. Vet Rec, 3(16-24): 578-580

[23]. Lidia, B. (2008): seroprevalence study of bovine brucellosis in central highland 0f Ethiopia,    DVM thesis, Jimma University, college of Agriculture and Veterinary Medicine, Jimma, Ethiopia

[24]. Mangen, M., Otte, M., Pfeiffer, J. and Chilonda, P. (2002): Bovine brucellosis in Sub-Saharan Africa; estimation of seroprevalence and impact on meat and milk off take potential,     Livestock   Policy Discussion Paper No 8, Food and Agriculture Organization, Livestock information and policy branch AGAL.

[25]. Molla, B. (1989): Seroepidimiological survey of bovine brucellosis in Arsi Region. DVM thesis,          FVM, Addis Abeba University Debrezeit, Ethiopia.

[26]. Morgan, W.J., MacKinnon, D.J., Lawson, J.R. and Cullen,G.A. (1996): The rose Bengal plate agglutination test in the diagnosis of brucellosis. Vet. Record, 85: 636-641.

[27]. Nakavuma, J. (1994): A serological survey of Brucella abortus in cattle and goats in the central           and Southern Regions of Uganda, MSc. Thesis.Faculty of veterinary Medicine, Makerere University, Kampala, Uganda.

[28]. Neilsen, K. (1990): The serological response of cattle immunized with Yersinia enterocolitica O: 9 or O:16 to Yersinia and Brucella abortus antigens in enzyeme immunoassays. Vet.         Immunol.Immunopathol.,24: 73-382. Vet. and Animal Sci., 29: 1157-1162.

[29]. OIE (2004): world organization for animal health. Bovine brucellosis, in manual standard for diagnostic tests and vaccines 5thed, paris. Pp 242-262.

[30]. OIE, (2008): Bovine brucellosis manual of Diagnostic Tests and Vaccines for Terrisrerial         animals, office International Des Epizooties,Pp 409-435.

[31]. Omer, M.K., Skjerve, E., Holstand, G., Woldehiwot, Z. and Macmillan,A.P.(2000): Prevalence            of antibodies to Brucella species in cattle, sheep, goats horses and camels in the state of            Eritrea, influence of husbandry system. Epigemol. Infect. 125(2): 447-453.

[32]. Radostits, M., Gay.,Hinchcliff, W. and Constable, D. (2007): Veterinary medicine. A text of    disease of cattle, horses, sheep, goats and pigs, 10th ed. Saunders, Philadelphia, Pa, USA.

[33]. Rashid , M. (1993) : reproductive wastage in cattle due to bovine brucellosis proceeding of the 4thNational Livestock improvement conference, 13-15th November 1991, Institute of Agricultural research, Addis Abeba, Pp 270-272.

[34]. Robert, S.J. (1986): Veterinary obstetrics and Genital diseases. 2nded.CBS publisher and           Distributers, Pp 107.

Shiferaw, A. (1994): Seroepidimiological study of bovine brucellosis in around Bahir Dar, DVM     thesis, FVM, Addis Abeba ,Debrezeit, Ethiopia.

Tadele, T. (2004): Seroprevalence of bovine brucellosis and its puplic health significance in selected Zone, western Ethiopia.

[35]. Taddese, Y. (2003): A survey of bovine in selected areas of North Gonder Zone, DVM Thesis,           FVM, AAU, DebreZeit, Ethiopia.

[36]. Teferi, D., Asmamaw, D. and Reta, D. (2011): Brucellosis and Some reproductive problems of            Indigenous Arsi Cattle in selected Arsi zones of Oromia Regional state, Ethiopia, Glob    Vet, 7(1): 45-53.

[37]. Thrusfield, M. (1995): Sampling in veterinary Epidemiology, 2nd ed. B;lackwell science, Oxford,         Pp251-281.

[38]. Tujuba, J., B. Kelay., M. Bekana., S. Teshale., H. Gustafason and H. Kindah. (2006): Epidimiological study of bovine brucellosis in three Agro-ecological areas of central    Oromia, Ethiopia.

[39]. Walker, L. (1999): Brucella in dwright, C.H change,   veterinary microbiology, Massachusetts,             Black well science.

[40]. WHO. (1986): FAO-WHO expert committee on brucellosis. 6th report, world health organization Technical             Report series    740, Geneva, Pp 27-66.